L753

Test CODE NAME

L753

Acid-fast (Mycobacteria) Smear and Culture With Reflex to Identification (AFB)

Methodology: Concentrated smears are stained with auramine/rhodamine and read by fluorescence microscopy. Broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported
Performed: Varies
Reported: 45-58 days
Specimen Required:

Patient Preparation: None required.

 

Collect: Sputum or respiratory aspirate - 5 ml  in a Sterile container with tight screw-cap seal OR  Green-top (sodium heparin) tube - 10 mL whole blood OR Body fluid (eg, gastric aspirate/lavage, urine, pleural, pericardial, chronic peritoneal dialysate) - 50 ml ,CFS, bone marrow (or as much as possible) in a Sterile container with tight screw-cap seal OR  Stool  - 10 ml in Para Pak White OR Biopsy of skin in a Sterile container with tight screw-cap seal.

Specimen Preparation: First morning sputum (not saliva) (three separate specimens from three separate days are recommended), fasting gastric aspirate, induced sputum, whole blood, tissue, biopsy, bronchial aspirate, urine, skin, cerebrospinal fluid (CSF), bone marrow, body fluid, stool.

Storage/Transport Temperature: Refrigerated. If sample is to be split for other tests, specimen should be divided at the time of collection so that each portion is transported at the appropriate temperature.

Unacceptable Conditions: Inadequate quantity of specimen, specimen received in expired transport media or inappropriate transport device; specimen received after prolonged delay (usually >72 hours); specimen received after leaking out of transport container into the specimen bag (trach-suction devices will often leak if the cap with tubing is not removed and replaced by a solid cap); syringe with attached needle; unlabeled specimen or name discrepancy between specimen and request label; lithium heparin tube; specimen submitted with eSwab device.

Stability:  Refrigerated: 3 days

 

Note:   * This test is approved for all states. * 

CPT Code(s): 87206, 87116
Laboratory Corporation of America® Holdings and Lexi-Comp Inc.

Laboratory Corporation of America® Holdings and Lexi-Comp Inc.

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