Test CODE | NAME |
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L753
Acid-fast (Mycobacteria) Smear and Culture With Reflex to Identification (AFB)
Methodology: | Concentrated smears are stained with auramine/rhodamine and read by fluorescence microscopy. Broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported |
Performed: | Varies |
Reported: | 45-58 days |
Specimen Required: |
Patient Preparation: None required.
Collect: Sputum or respiratory aspirate - 5 ml in a Sterile container with tight screw-cap seal OR Green-top (sodium heparin) tube - 10 mL whole blood OR Body fluid (eg, gastric aspirate/lavage, urine, pleural, pericardial, chronic peritoneal dialysate) - 50 ml ,CFS, bone marrow (or as much as possible) in a Sterile container with tight screw-cap seal OR Stool - 10 ml in Para Pak White OR Biopsy of skin in a Sterile container with tight screw-cap seal. Specimen Preparation: First morning sputum (not saliva) (three separate specimens from three separate days are recommended), fasting gastric aspirate, induced sputum, whole blood, tissue, biopsy, bronchial aspirate, urine, skin, cerebrospinal fluid (CSF), bone marrow, body fluid, stool. Storage/Transport Temperature: Refrigerated. If sample is to be split for other tests, specimen should be divided at the time of collection so that each portion is transported at the appropriate temperature. Unacceptable Conditions: Inadequate quantity of specimen, specimen received in expired transport media or inappropriate transport device; specimen received after prolonged delay (usually >72 hours); specimen received after leaking out of transport container into the specimen bag (trach-suction devices will often leak if the cap with tubing is not removed and replaced by a solid cap); syringe with attached needle; unlabeled specimen or name discrepancy between specimen and request label; lithium heparin tube; specimen submitted with eSwab device. Stability: Refrigerated: 3 days
Note: * This test is approved for all states. * |
CPT Code(s): | 87206, 87116 |
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