M305

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Test CODE NAME

M305

Lyme Disease (Borrelia Species by PCR)

Methodology: Quantitative Polymerase Chain Reaction
Performed: Varies
Reported: 2-5 days
Specimen Required: Collect: Whole blood yellow top tube (ACD solution A), UroSwab® (males and females), synovial fluid, ticks
Specimen Preparation: 
Yellow top tube (ACD solution A): 
•Immediately after blood collection, invert the tube(s) gently 8-10 times to ensure mixing of anticoagulant with blood to prevent clotting.  
•Do not shake tube(s). 
•Do not centrifuge specimen.  
Storage/Transport Temperature: Stable at room temperature.  
•Completely fill the tube whenever possible to eliminate dilution from the anticoagulant and ensure the proper blood-to-anticoagulant ratio. 
UroSwab®: 
Storage/Transport Temperature: Stable at room temperature.  
Synovial Fluid in a Sterile Container: min 1 ml  
•Please list specimen source on the container and test requisition form. 
•Do not use the yellow top (ACD solution A) blood collection tubes. Storage/Transport Temperature: Stable at room temperature.  
Tick: in Clean non-additive container or zip-lock bag: 
•Ticks are consumed during the extraction process and therefore cannot be returned after testing. 
•Turnaround time for tick testing is typically 3-5 days but may vary. Storage/Transport Temperature: Stable at room temperature.  
•Ticks can be submitted alive or dead.  
•Do not place in alcohol, burn, or place on tape.
Stability: Room Temperature: 24-48 hours.
 

NOTE: * This test is approved for all states. * 
Performing Laboratory 
MDL (Molecular Diagnostics Laboratories)
CPT Code(s): 87476